Table of content

Onderstepoort Journal of Veterinary Research

ISSN/EISSN: 00302465 22190635
Subject: Animal Sciences
Publisher: AOSIS OpenJournals
Country: South Africa
Language: English
Start year 2003
Publication fee: No --- Further Information

Journal homepage at publisher site


Table of content: 2010 volume:71 issue:4

Article
High infection rates of the tick <i>Hyalomma anatolicum</i> anatolicum with <i>Trypanosoma theileri</i>

Authors: A.A. Latif --- M.A. Bakheit --- Amna E. E. Mohamed --- E. Zweygarth
DOI: 10.4102/ojvr.v71i4.228
Pages: 251-256
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Abstract

<span style="font-family: arial,helvetica;">A crossbred calf (3 months old) obtained from a farm where regular control of ticks was practised and found to be free of blood parasites was inoculated with 20 ml pooled blood collected from four field cattle which had very low <em>Trypanosoma theileri</em> parasitaemias (one parasite per 70 &micro;l blood as determined by the haematocrit centrifugation technique). <em>Trypanosoma theileri</em> was present in the blood 6 days after injection and a peak parasitaemia of 42 parasites per 70 &micro;l blood was recorded by day 12. <em>Hyalomma anatolicum anatolicum</em> nymphs were applied on the ears of the calf on day 8 and they dropped engorged by days 13 and 14. The resulting adult ticks were examined for the presence of <em>T. theileri</em> by severing a leg and making a smear of the clear haemolymph which exuded from the wound. The smear was fixed in methanol and stained with Giemsa stain. The infection rate with <em>T. theileri</em> in the ticks was 43.3 % (26 out of 60). The intensity of infection was very high and various developmental stages of the flagellates were observed (epimastigotes, sphaeromastigotes, trypomastigotes and other intermediate stages). The haemolymph from 12 ticks was also collected in tissue culture medium and the trypanosomes survived for 25 weeks before eventually dying. The results demonstrated unequivocally the high vectorial capacity of the tick <em>H. a. anatolicum</em> for <em>T. theileri</em>.</span>

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Article
The point prevalence of gastro-intestinal parasites in calves, sheep and goats in Magadi division, south-western Keny

Authors: M.W. Maichomo --- J.M. Kagira --- T. Walker
DOI: 10.4102/ojvr.v71i4.229
Pages: 257-261
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Abstract

<span style="font-family: arial,helvetica;">Helminths cause great economic loss in livestock in Africa, and can be categorized as either direct or indirect losses. Arid and semi-arid lands (ASAL) in Kenya comprise 71 % of total land area and harbour the largest population of cattle, sheep and goats. However, little information on the distribution and impact of gastro-intestinal (GIT) parasitism in these animals is available. This survey was conducted to establish the prevalence of GIT parasites infecting calves, sheep and goats and their relative importance in Magadi division, which is semi-arid. Faecal samples were obtained directly from the rectum of 109 calves, 133 goats and 20 sheep and submitted to the laboratory for faecal worm egg counts, and coccidial oocysts examination using a modified McMaster method. The significance of differences in mean egg count per gram (epg) between animal species and herds (farms) were assessed using analysis of variance. The overall prevalence of nematodes in the calves, sheep and goats was 69.2 %, 80 % and 82 %, respectively. About 10 % of sheep and goats had epgs higher than 1 000, the remainder having light to moderate infections. The overall prevalence of coccidial oocysts in calves, sheep and goats was 30 %, 44 % and 45 %, respectively. Poor productivity in ASAL areas, where nutrition is often poor, is likely to be pronounced in the presence of parasite infections. These findings indicate that viable internal parasite control should be implemented in the study area in order to increase the productivity of the livestock there.</span>

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Article
General morphology of the oral cavity of the Nile crocodile, <i>Crocodylus niloticus</i> (Laurenti, 1768). II. The tongue

Authors: J.F. Putterill --- J.T. Soley
DOI: 10.4102/ojvr.v71i4.230
Pages: 263-277
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Abstract

<span style="font-family: arial,helvetica;">The heads of nine 2.5 to 3-year-old Nile crocodiles (<em>Crocodylus niloticus</em>) were obtained from a commercial farm where crocodiles are raised for their skins and meat. The animals from which these specimens were obtained appeared clinically healthy at the time they were slaughtered. A description of the macroscopic and microscopic features of the tongue of the Nile crocodile is presented and the results are compared with published information on this species and other Crocodylia. The histological features are supplemented by information supplied by scanning electron microscopy. Macroscopic features of interest were the dome shaped structures grouped in a triangular formation on the posterior two-thirds of the dorsum of the tongue. These structures were identified by light microscopy to contain well-developed branched, coiled tubular glands and associated lymphoid tissue. Other histological features included a lightly keratinised stratified squamous surface epithelium supported by a thick layer of irregular dense fibrous connective tissue. Deep to this region was a clearly demarcated adipose tissue core with a dense mass of striated lingual musculature. Localised thickenings were present in the epithelium which were associated with ellipsoid intra-epithelial structures resembling taste buds.</span>

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Article
Ixodid ticks on dogs in southern Mozambique

Authors: L. Neves --- Sonia Afonso --- I.G. Horak
DOI: 10.4102/ojvr.v71i4.231
Pages: 279-283
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Abstract

<span style="font-family: arial,helvetica;">The objective of this study was to determine the species and distribution of ticks infesting domestic dogs in southern Mozambique. To this end 89 collections were taken from dogs and the geographic coordinates of the localities at which they were made noted. Fifty-eight of these collections were from animals in the city of Maputo and 16 from animals at rural settlements close to Maputo. A further 15 collections were made from dogs in rural environments north of Maputo. Ten ixodid tick species, of which one was only identified to genus level, were recovered. <em>Rhipicephalus sanguineus</em> was the most numerous of the ten species, and its prevalence and intensity of infestation were significantly higher on city dogs than on rural dogs (P&lt; 0.01), whereas the converse was true for <em>Haemaphysalis leachi</em> (P&lt; 0.01). Including the city of Maputo, the exact localities at which nine tick species were collected, were recorded.</span>

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Article
Effect of urea-molasses block supplementation on grazing weaner goats naturally infected with gastrointestinal nematodes

Authors: R.M. Waruiru --- J.W. Ngotho --- M.N. Mutune
DOI: 10.4102/ojvr.v71i4.248
Pages: 285-289
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Abstract

<span>The influence of feeding urea-molasses blocks (UMB) on growth and gastrointestinal (GI) nematode parasitism of weaner goats grazing the same pasture was investigated on a farm in Nyandarua District, Kenya. Thirty female Small East African goat kids at an average age of 5 months were initially treated with albendazole orally (5 mg kg<sup>-1</sup> body mass) and randomly assigned into one of two groups: group I were fed UMB prepared using a cold process and group II kids (controls) received no block supplementation (NBS). The UMB were given in the evening when the animals returned from grazing and were consumed during the night at a rate of 95.0 g head<sup>-1</sup> day<sup>-1</sup>. Supplementation was undertaken for 3 consecutive months from July to September 2001 and January to March 2002. Body mass of the kids and faecal egg counts were measured monthly and larval cultures were performed on positive faecal samples of kids of each group. Five goats from each group were randomly selected for slaughter and total counts and identification of worms at the end of June 2002. Significant differences (P &lt; 0.05) were found in cumulative mass gains of kids in group I from September compared with those in group II. On termination of the study kids in group I had gained an average of (<span style="text-decoration: underline;">+</span> SD) 20.4 &plusmn; 1.4 kg while those in group II had gained 11.8 <span style="text-decoration: underline;">+</span> 1.1 kg. From January 2002, faecal egg counts of the kids in the UMB group differed significantly (P &lt; 0.05) from those of the NBS group and at slaughter, the mean (<span style="text-decoration: underline;">+</span> SD) worm counts for the UMB group was 482 <span style="text-decoration: underline;">+</span> 299 while that of the NBS group was 1 302 <span style="text-decoration: underline;">+</span> 410. In all the goats, <em>Haemonchus contortus</em> was the predominant nematode recovered. These results indicate that UMB had significant effects in the control of GI nematode parasitism and enhanced growth of the young goats.</span>

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Article
Parasites of domestic and wild animals in South Africa. XLV. Helminths of dairy calves on dry-land Kikuyu grass pastures in the Eastern Cape Province

Authors: I.G. Horak --- Ursula Evans --- R.E. Purnell
DOI: 10.4102/ojvr.v71i4.249
Pages: 291-306
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Abstract

<span>Successive pairs of approximately 4-month-old Friesland bull calves, raised under worm-free conditions, were exposed to helminth infection for 14 days on dry-land Kikuyu grass pastures at 28-day to monthly intervals, on a coastal farm in a non-seasonal rainfall region of the Eastern Cape Province. With the exception of one pair of calves exposed for 28 days, this procedure was repeated for 28 consecutive months from December 1982 to March 1985. The day after removal from the pastures one calf of each pair was slaughtered and processed for helminth recovery and the other 21 days later. Both members of the last four pairs of calves were killed 21 days after removal from the pastures. <br />Sixteen nematode species were recovered from the calves, and infection with <em>Ostertagia ostertagi</em> was the most intense and prevalent, followed by <em>Cooperia oncophora</em>. The calves acquired the greatest number of nematodes from the pastures from June to October of the first year and from June to August of the second year of the survey. Few worms were recovered from the tracer calves examined from November or December to March or April in each year of the survey. The seasonal patterns of infection with <em>Cooperia</em> spp., <em>Haemonchus placei, Nematodirus helvetianus, Oesophagostomum</em> spp., <em>O. ostertagi</em> and <em>Trichostrongylus axei</em> were all similar and were negatively correlated to atmospheric temperature and evaporation. Slight to moderate arrest in the development of fourth stage larvae occurred from July to September in <em>Cooperia</em> spp., April to July in <em>H. placei</em>, and August to October in <em>O. ostertagi</em> and <em>Trichostrongylus</em> spp. during the first year of the survey. Too few worms were present in the second year to determine a seasonal pattern of arrest. Species survival during the hot and windy summer months appeared to be achieved via a combination of arrested larval development and an ageing residual population of adult worms in the host, and a small extant population of infective larvae on the pastures.</span>

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Article
Seasonal prevalence of bovine trypanosomosis in a tsetse-infested zone and a tsetse-free zone of the Amhara Region, north-west Ethiopia

Authors: T. Cherenet --- R.A. Sani --- J.M. Panandam --- S. Nadzr --- et al.
DOI: 10.4102/ojvr.v71i4.250
Pages: 307-312
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Abstract

<span>During a period of four consecutive years, trypanosomosis surveys were conducted in a tsetse-infested and tsetse-free area of the Amhara Region of north-west Ethiopia. In each study area randomly selected communal cattle were sampled and their blood was investigated using parasitological diagnostic methods. At the same time the population of biting flies was sampled. The monthly average prevalence of trypanosome infections in cattle did not differ significantly between study areas. In both study areas, the prevalence of trypanosome infections was highest during the long rainy season. Trypanosome infections were mainly due to <em>Trypanosoma vivax</em> and they significantly reduced the average packed cell volume and the body condition of the animals. The monthly prevalence of infection was correlated with the density of biting flies, such as Tabanidae and <em>Stomoxys</em> spp., in the preceding month suggesting an important role of mechanical transmission in the epidemiology of trypanosomosis in both areas.</span>

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Article
Striped mice, Rhabdomys pumilio, and other murid rodents as hosts for immature ixodid ticks in the Eastern Cape Province

Authors: T.N. Petney --- I.G. Horak --- D.J. Howell --- S. Meyer
DOI: 10.4102/ojvr.v71i4.251
Pages: 313-318
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<span>Striped mice, <em>Rhabdomys pumilio</em>, were trapped over a period of 17 months in the Thomas Baines Nature Reserve, and placed in cages, over water, until all the ticks they harboured had detached. The mice were then returned to the reserve. Four ixodid tick species were recovered from the mice of which the larvae and nymphs of <em>Rhipicephalus follis</em> and <em>Rhipicephalus simus</em> were the most numerous. Most larvae of <em>R. follis</em> detached from mice trapped from March to July, and most nymphs in March and from June to September. Most larvae of <em>R. simus</em> detached from mice trapped from December to March, and most nymphs from January to March and during May and June. Seven ixodid tick species were collected from striped mice, house rats, <em>Rattus rattus</em>, vlei rats, <em>Otomys</em> spp. and <em>Praomys</em> sp. captured in the vicinity of human dwellings or animal holding facilities in the Grahamstown district. The striped mice captured in the Thomas Baines Reserve harboured considerably larger numbers of ticks than any of the rodent species in the more urbanized localit</span>

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Article
Preliminary survey of ticks (Acari : Ixodidae) on cattle in northern Sudan

Authors: D.A. Salih --- S.M. Hassan --- A.M. El Hussein --- F. Jongejan
DOI: 10.4102/ojvr.v71i4.252
Pages: 319-326
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Abstract

<span>In a cross sectional survey conducted during the period June 2001 to July 2002, the geographical distribution of ticks on cattle in the Sudan was determined. Seventeen locations were surveyed from Northern, Central, Eastern, Western, Blue Nile and White Nile Provinces. Total body collections of ticks were made from 20 cattle at each location. Four tick genera and 11 species were identified. The tick species collected included <em>Amblyomma lepidum, Amblyomma variegatum, Boophilus decoloratus, Hyalomma anatolicum anatolicum, Hyalomma dromedarii, Hyalomma impeltatum, Hyalomma marginatum rufipes, Hyalomma truncatum, Rhipicephalus evertsi evertsi, Rhipicephalus sanguineus</em> group and <em>Rhipicephalus simus simus</em>. Major ecological changes have occurred due to extensive animal movement, deforestation, desertification and establishment of large mechanized agricultural schemes. These factors have certainly affected the distribution of ticks and tick-borne diseases in the Sudan. The absence of <em>A. variegatum</em> and <em>A. lepidum</em> in northern Sudan was not surprising, since these tick species are known to survive in humid areas and not in the desert and semi-desert areas of northern Sudan. The absence of <em>B. annulatus</em> in northern and central Sudan is in accordance with the finding that this tick species is restricted to the southern parts of the central Sudan. The presence of <em>H. anatolicum anatolicum</em> in Um Benin in relatively high abundance is an interesting finding. The present finding may indicate that the southern limit of this species has changed and moved southwards to latitude 13<sup>o</sup> N. It is concluded that major changes in tick distribution have taken place in the Sudan</span>

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Article
Larval biology of Rhipicephalus (Boophilus) decoloratus (Acarina : Ixodidae) in Free State Province, South Africa : research communication

Authors: M.S. Phalatsi --- L.J. Fourie --- I.G. Horak
DOI: 10.4102/ojvr.v71i4.253
Pages: 327-331
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<span>The objective of this study was to determine certain aspects of the biology of <em>Rhipicephalus (Boophilus) decoloratus</em> larvae under laboratory and field conditions. Larvae allowed 48 h to select a vertical questing substrate preferred 90 cm rods in length to those of 60 or 30 cm, while in a separate experiment migration from rods 5 cm or 25 cm in length to rods 45 cm in length continued between 48 h and 72 h after larval release. Hatching of the larval progeny of engorged female ticks exposed to ambient field temperatures during the period June to August, occurred synchronously during the third or fourth week of November. With a single exception, larvae that hatched during November and between April and July survived for 38 days or longer, while those that hatched from December to March survived for 31 days or less. Questing larvae were present on vegetation throughout the year, with most being recovered during January and February. Parasitic larvae were present on cattle from October to May with most being collected during January and February.</span>

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Article
Progression towards endemic stability to bovine babesiosis in cattle introduced onto a game ranch : research communication

Authors: Assefa Regassa --- B.L. Penzhorn --- N.R. Bryson
DOI: 10.4102/ojvr.v71i4.254
Pages: 333-336
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<span>An opportunity to study progression toward endemic stability to <em>Babesia bigemina</em> arose when cattle were reintroduced onto a game ranch in 1999 after an absence of three years. The study was conducted between August 2000 and June 2001. The unvaccinated breeding cows were sampled only once. Calves born during October 1999 were initially vaccinated against <em>B. bigemina</em> and <em>Babesia bovis</em> at the age of 4 months and were then bled at 10, 17 and 20 months of age. Calves born during 2000 were bled at 7 and 8 months of age. Sera were collected from all the cattle sampled and later tested for antibodies against <em>B. bigemina</em> and <em>B. bovis</em> using the indirect fluorescent antibody (IFA) test. Although endemic stability to <em>B. bigemina</em> had not been achieved at Nooitgedacht 2 years after resumption of cattle ranching, the high seroprevalence in the unvaccinated 8- month-old calves suggested that the situation was approaching stability and that calf vaccination against bovine babesiosis was not required. Tick control should therefore be restricted to prevent excessive tick worry. Only vaccinated cattle were positive to <em>B. bovis</em> and it was concluded that the parasite was absent from the ranch.</span>

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Table of content: 2010 volume:71 issue:4