Ensuring the quality management of the water source in the hydrographic basin Someş-Tisa, in the acception of the Water Framework Directive 2000/60/EC, to reach to the good ecological status, involves the establishment of integrated monitoring and of some action plans for those sources that generate priority/priority dangerous substances (list I/ list II) in order to eliminate/decrease the pollution of the aquatic environment. The results of the monitoring put into light the areas of industrial/human activity generating chemical compounds under the incidence of EU Directive 2006/11/EC, replacing EU Directive 76/464/EC (the pollution due to discharges of dangerous substances in aquatic environment), as well as the level of pollution induced by them. From the analysis of the data base, the constitution at the level of the hydrographic basin Someş-Tisa, realized in concordance with the requirements of the legislation in the area of the management of the water source, the way of action and the measures needed to reach the level of compliance with the terms established in the Position Document between Romania and European Commission about Chapter 22 - Environment was adopted. The concreting of the diligences to comply with the EU Directive 2006/11/EC shall be realized in the report to elaborate at the end of the period of transition for the substances from List I (31.12.2009), as well as by applying the Management Plan of Someş-Tisa River Basin.

dangerous substances --- monitoring --- action plans --- evaluation

In this study we evaluated the effects of a standard rotifers/Artemia (control) diet and a copepod (Centropages typicus) based diet during Amphiprion clarkii larviculture. On day 11 post hatch, larvae fed C. typicus showed better survival and growth compared to those fed a standard rotifer/Artemia diet (87±2 vs 41±2%; 6.8± 0.2mm vs 6.1± 0.2mm; 5.9±0.3mg vs 4.5± 0.3 mg, respectively). Further molecular analysis have been carried out on 5 and 11 days post hatch larval samples to clarify the efficiency of a copepod based diet respect to control. In particular, the peroxisome proliferator activated receptors (PPAR-α, -β) gene expression, which are involved in several biological processes such as lipid metabolism, the retinoic acid receptor γ (RAR γ ), involved in vitamin Ametabolism and the heat shock protein 70 (HSP70), a biomarkers of cellular stress, were analyzed by real-time PCR during the larval development. The results obtained are related to fatty acid composition of live preys used. The superiority of a Centropages typicus copepod diet respect to a standard rotifers - Artemia one has been demonstrated; these results are essential to improve captive production of A. clarkii through a closed system and in turn to preserve natural stocks.

copepods --- fatty acids --- clownfish --- Artemia --- rotifers

Several studies have been conducted to detect the direct effect of inhibiting the aromatase activity, the rate limiting enzyme that converts androgens to estrogens needed for ovarian differentiation in fish to overcome the immediate need for a more environmentally friendly substitute of methyl testosterone. Cyclooxygenase (COX)-inhibitors are potent and irreversible inhibitors of the COX pathway and since studies on human breast cancer cells shows that they decrease aromatase messenger ribonucleic acid (mRNA) expression at the transcriptional level we tested the hypothesis of possible aromatase inhibition by the non-selective COX-inhibitors in fry fish tilapia. The effects of supplementation of COX-inhibitors (diclofenac and ibuprofen) in the diets of tilapia on growth rate, mortality and sexual differentiation have been studied. 20 Genetically females (XX) (O. niloticus) larvae were stocked in triplicates in a closed system and each were given control diet (C group) and control diet supplemented with (10 mgKg^-1) diclofenac (1% diclofenac group), (5 mgKg^-1) ibuprofen (0.5% ibuprofen group), and (5 mgKg^-1) (0.5% diclofenac group) respectively for 4 weeks. After the 4^th week all diets were changed to control diet. At the end of 12-weeks, no significant differences were found in growth rate (GR) between diets (p>0.05). Mortality ranged from 1.67% +- 2.89 (SD, n=3) in control group to 58.3% +- 14.4 (SD, n=3) in the 1% diclofenac group during the experimental feeding and from 6.67+-2.89 (SD, n=3) to 63.3%+-10.4 (SD, n=3) at the end of 12-weeks period. 7% In the control group, 36% in the 1% diclofenac group, 17% in the 0.5% ibuprofen group, and 22.2% in the 0.5% diclofenac group respectively never produced egg during the entire experimental period. Macroscopically all the nonspawning fish in the experimental groups were females with apparently larger ovaries and full of eggs compared to control. Microscopically they were full of apparently normal eggs with morphology similar to those of control. Postulated mechanisms of action of the supplemented COX-inhibitors are discussed. Based on the above it can be concluded that the use of COX-Inhibitors during the crucial period could modulate aromatase activity and affect reproduction in Nile tilapia.

COX-inhibitors --- Tilapia --- aromatase --- sexual differentiation

Prostate sampled from three nutria males was processed for histological examination. It was found that, unlike terrestrial mammals, the capsula and conjunctive trabeculae not contain smooth muscle cells, so they do not participate in the evacuation of the content from gland during ejaculation. Glandular tubes from prostate of nutria present own-developed muscularis, similar to the arteries media, which allows an evacuation of secretions from each tube in part, so that evacuation of the contents of nutria prostate during ejaculation is made under a greater pressure than in terrestrial mammals. This particular mode of evacuation of prostate secretions appears to be a functional adaptation due to the fact that the nutria male performs the sexual act also in water, where conditions are totally different from those on land.

Nutria --- prostate --- muscular component

A fish population from a given geographical area can be characterized in morphological terms by means of somatic measurements. Seeing that Romanian literature does not provide information about common minnow (Phoxinus phoxinus) population from Banat Hidrografic Area, Timis Hidrografic Basin, we launched a number of researches on external morphology to reveal the conformation body characters. Investigated body segments were: total body length (Tl), standard body length (Sl), head length (lc), snout length (snl), caudal peduncle length (lpc), maximum body depth (H), minimum body depth (h) and body weight (w). Making an analysis of average for total body length for common minnow (Phoxinus phoxinus) females and males, we observed that the females body average length was 7.1 ± 0.66 cm and the average length of males was 4.47 ± 0.15 cm. Average body weight obtained for females was 4.31 ± 0.41 g, and for males was 0.98 ± 0.08 g. The standard length of common minnow (Phoxinus phoxinus) females is 84.78 % of the total body length, and head length (1.41 ± 0.17 cm) is 19.85 % of the total body length. For common minnow (Phoxinus phoxinus) males the standard body lenght is 83.44 % of the total body length and head length (0.93 ± 0.03 cm) is 20.80 % of the total body length.

common minnow --- Phoxinus phoxinus --- river --- somatic measurements