In this study five species of probiotic bacillus as bacterial blend under the commercial title ofProtexin aquatic were used for bioencapsulation of Artemia urmiana (Gunther, 1899). This experimentwas conducted in a completely random design. A. urmiana naupli I was used as a vector to carryprobiotic bacillus to digestive tract of Huso huso (Linnaeus, 1758) larvae. Nauplii with threeconcentrations of bacteria, 1×10⁵, 2×10⁵ and 3×10⁵ bacteria per milliliter in suspension of broth for 10hours were bioencapsulated and beluga larvae were fed by them. Beluga larvae were fed 50 percent oftheir body weight for 6 times a day. The control treatment was fed by unbioencapsulated artemia nauplii.The results indicated that the probiotic bacillus could influence on growth and feeding parameters inbeluga larvae. The final body weight and specific growth rate (SGR) for weight in experimentaltreatments had significant difference in comparison to control treatment (P0.05). In experimentaltreatments the Food efficiency was increased significantly in comparison with control. The probioticbacillus had significant positive effects on conversion efficiency ratio (CER), Daily growth coefficient(DGC) and average weight gain (AWG) in comparison to control treatment (P<0.05). Also the relativefood intake (RFI) significantly decreased (P<0.05) while Protein gain (PG) and Energy retained as protein(PD.KJ day^-1) significantly increased (P<0.05).

Probiotic --- bioencapsulation --- Artemia nauplii --- specific growth rate --- relative food intake

A rapid expansion in world aquaculture has taken place in recent years due to its highestpotential to answer to the consumers demands on healthy food products. The application of biosecurityin aquaculture production systems and the safety of fish and fish products, in the context of climatechanges, requires investigations of the production systems and solutions for processing andpreservation technologies of fish products. PCR methods will be developed using the procedure ofgenes fragments amplification for Clostridium botulinum van Ermengem, 1896 species producingtoxins A, B, E and F from isolates of C. botulinum using extract broth TGPY (culture medium used forproteolytic strains) and TPGYT (culture medium used for non-proteolytic strains of C. botulinum). Theresults will contribute to the integral development of food safety management and the quality of fishon the fish chain focused on the Cyprinidae for humans consumption.

Aquaculture --- food safety --- PCR

In order to study the effects of storage media and time of storage on the viability ofunfertilized eggs of Prussian carp, Carassius gibelio (Bloch, 1782), the ova of this fish, outside the ovary(ex situ storage), were stored in Dettlaff extender (111.3 NaCl + 3.3 KCl + 2.1 CaCl2 + 23.8 NaHCO3)and PCACF (Prussian carp Artificial Coelomic Fluid) medium at 4°C and 21°C for 20, 30, 60 and 120 min.For mimicking of ovarian fluid and designing the PCACF medium, obtained ova from 15 female, werefiltered and coelomic fluid was separated for chemical composition analysis of ovarian fluid and thePC ACF medium was formulated. This study was done with 16 treatments and 1 control group in 3replicate. The results shown that maintain duration and storage medium had significant effect on eyedembryos rate, hatching success (P<0.05), but their effect on malformation of larvae was not significant(P>0.05). Also, temperature had significant effects on percentage of eyed embryos and hatching rate(P<0.05) but no significant effects on number of malformed larvae (P>0.05). Eyed embryo and hatchingrates decreased with increasing time. Interactions between time of storage and storage medium andtemperature and the resulting effects on eye embryos and hatching rates were significant (P<0.05).Highest eyed embryo and hatching rate between treatments was observed in PCACF, 20 min storage and4 °C (PCACF + 20 min + 4 °C) and lowest eyed embryo and hatching rates was observed in Dettlaff +120 min + 21 °C.

short-term storage ova --- artificial media --- Prussian carp

The aim of this study was to assess the effect of garlic (Allium sativum) on growth factors,some hematological parameters and body compositions in rainbow trout (Oncorhynchus mykiss). A totalnumber of 360 fish (average weight 20.88±0.25 g) was used. Fish were divided into four groups fed ondiets containing garlic in different levels; 10 g kg^-1, 20 g kg^-1, 30 g kg^-1 diet and the control group dietwas without garlic. The experiment extended for two months. The results showed that, weight gain andgrowth performance of O. mykiss significantly (p<0.05) increased in all groups fed on garlic. There wassignificant decreases of plasma glucose in fish fed on diets containing garlic. Mean values of total plasmaprotein increased significantly in all treatments when compared to control group (p<0.05). Proteincontent and ash contents in fish body were significantly higher in the group fed on diet containing 30 gkg^-1 diet of garlic than all other groups. Total lipids content in fish body decreased in treatments and itwas lower in fish fed on 30 g kg^-1 diet of garlic. The results of this study show that addition of garlicAllium sativum to fish diet can promote growth and improve fish health.

garlic (Allium sativum) --- growth factors --- hematological parameters --- body compositions --- rainbow trout (Oncorhynchus mykiss)