Effects of coelomic fluid on spermatozoa mobility trait, in sturgeons, have not been knownwell. This research aims to determine the content of inorganic (magnesium, sodium, potassium andcalcium) and organic ions (cholesterol and total protein) in coelomic fluid and to investigate the effects ofdifferent levels of coelomic fluid on some spermatozoa mobility traits (duration of sperm mobility and thepercentage of motile sperms) in Persian sturgeon brood stocks. Hence milts have been sampled fromseveral male brood stocks and have been mixed by coelomic fluid in different ratios (1 to 20, 15, 10, 7.5,5, 2.5, 1, 0.5, 0.33, 0.25, 0.20 and 0), then mobility traits have been measured by stereomicroscope.Results of this research show that, if coelomic fluid is added to sperm more than 2.5 times, all sperms ofPersian sturgeon brood stocks will perish and if coelimic fluid is added to sperm less than 2.5 times, theduration of sperm mobility and the percentage of motile sperms will increase significantly, comparing tocontroled group (which does not have coelimic fluid) (P<0.05). Besides, the maximum duration of spermmobility and percentage of motile sperms was respectively observed among those treated groups inwhich the ratio of coelomic fluid to sperm equaled to 1:0.33 and 1:0.5 (P<0.05).

Coelomic fluid --- Spermatozoa mobility trait --- Persian sturgeon

Swollen Hindgut syndrome (SHG) of black tiger shrimp Penaeus monodon Fabricius, 1798post larvae has been reported from Bangladesh shrimp hatcheries in recent years. At present SHG is abudding problem causing significant economic loss to the shrimp hatcheries in Bangladesh. Unlike therapid mortalities associated with viral disease such as white spot syndrome and yellow head virus,progression of SHG is gradual leading to low level mortalities without affecting swimming activity. Thesign of SHG are a bloated or swollen in hind gut area, with the posterior digestive tract convolutingthrough the last abdominal segment. This syndrome tends to occur at later PL stages, typically afterPL10. Two types of Vibrio spp. were isolated from the swollen hindgut syndrome post larvae, identified asVibrio harveyi (Johnson and Shunk 1936)and Vibrio alginolyticus (Miyamoto, Nakamura & Takizawa1961). Among these V. alginolyticus was dominant to V. harveyi. The bacterial isolates showed sensitiveto oxytetracycline (OTC), norfloxacin and ciprofloxacin and resistant to penicillin, ampicillin andamoxycillin. The luminous V. harveyi showed resistant to many antibiotics and susceptibility to only twodrugs. The cause of swollen hindgut syndrome (SHG) was probably bacterial infections and poor waterquality, possibly heavy metal i.e. iron, the presence of toxic substances from chemical prophylactics andlow quality or diseased nauplii.

Swollen hindgut syndrome --- Vibrio spp. antibiotic --- Penaeus monodon

The wide distribution and high tolerance to physical and chemical factors makes it possible forEphemeroptera to be used as a bioindicators. The objective of this paper was to bring some new informationregarding the distribution of the ephemera species in western Romania, together with the analysis of speciesdistribution in relation to certain physical-chemical parameters. The samples were taken from the three riverbasins of the Aninei Mountains (Bârzava, Caraș and Nera), totalizing a number of 27 sites located on thetributary and on the main course of the rivers. We identified a number of 14 species, the most commonbeing Ecdyonurus venosus, Ecdyonurus torrentis, Bäetis rhodani and Serratella ignita. The statisticalinterpretation, related to physicochemical factors showed a decisive effect of the water temperature, ofdissolved oxygen, magnesium, nitrate and cyanide, concerning the distribution of some common species ofmayflies.

Ephemeroptera --- diversity --- Aninei Mountains --- Bârzava --- Nera --- Caraș

The aim of this study was evaluation the effects of supplemented diets by levamisole andEchinacea purpurea (Moench, 1794) extract on growth and reproductive parameters in angelfish,Pterophyllum scalare (Lictenstein, 1823). 54 Uniform angelfish (average weight 3.8±0.29 g) wereselected. The fishes were fed by three diets which included commercial extruder diet (control),commercial extruder diet supplemented by 0.25 ppt levamisole (T1) and commercial extruder dietsupplemented by 0.25 ppt Echinacea purpurea extract (T2). The experiment was conducted for 3 months.The weight gain in T2 was significantly higher than other groups (p<0.05). There was no significantdifference (p>0.05) in fecundity and hatching rate among groups but the best survival of yolk-sacabsorption period was found in group fed supplemented diet by Echinacea extract (p<0.05). This studyindicates that growth performance can be improved by dietary Echinacea extract supplementation andlarval survival can be promoted by it.

supplemented diets --- levamisole --- Echinacea purpurea --- growth --- reproductive parameters --- angelfish (Pterophyllum scalare)

The anthropic impact, which takes various forms, changes the water quality parameters anddisturb the living environment of benthic macroinvertebrates. The present study aims to track the waypollution sources influence the macrozoobenthic community. Collecting quantitative samples, at differentseasons, from Mureș River and Cărămidăriei Lake, within the area of Lunca Mureșului Nature Park beforeand after each major pollution source, shows the way pollution affects, both qualitatively andquantitatively, the community of benthic invertebrates. 9 groups of benthic invertebrates were identified,their density, abundance and frequency undergoing changes from one station to another and from oneseason to another. When the river flows through the park, the benthic community reflects strong waterquality degradation of Mureș River. The river, naturally flowing on a long distance allows, through theself-purification process, an improvement in water quality emphasized by the occurrence of somesensitive groups to pollution which disappear completely once the tributary stream Mureșelul and othernon-purified waters from the town of Pecica flow into the river. The seasonal dynamics of dominantgroups (Oligochaeta, Diptera-Chironomidae) suggests a similarity, recording high values within theautumn-winter time interval.

benthonic macroinvertebrates --- water quality --- diversity

EDTA is a chelating agent used deliberately in various fields (pulp and paper industry,detergents industry, food industry, medicine, biomedical labs) in order to sequester metal ions whichhave harmful effects in many processes as well as in obtaining of many products. Taking as starting pointthe EDTA property to form metal-EDTA complexes we decided to test its effectiveness in mobilizing of thecontaminant metal ions, especially cadmium from tissue of Prussian carp specimens subjected to chronicpoisoning with cadmium acetate. At the same time we investigated the EDTA ability to reduce the knowncadmium antagonism exhibited vis-à-vis some essential macro-and microminerals.

chronic cadmium intoxication --- EDTA --- fresh water fish --- mineral micronutrients --- mineral macronutrients.