Siberian Journal of Life Sciences and Agriculture (Dec 2024)
ASSESSMENT OF MORPHOGENETIC PARAMETERS OF THE GENUS GLEDITSIA IN ARID CLIMATE
Abstract
Background. Representatives of the genus Gleditsia are valuable trees in arid climates, as this genus has a high degree of drought resistance and is tolerant to saline soil conditions. The purpose of this work is a comparative analysis of the morphogenetic parameters of seedlings with different natural ranges introduced in arid conditions: gledichia texana (G. texana), gledichia tricolor (G. triacanthos), gledichia tricolor (G. triacanthos, f. inermis), gledichia Caspian (G. caspica). Materials and methods. Object of study: seedlings of some species of the genus Gleditsia: G. texana, G. triacanthos, G. caspica, which grow on the territory of the Federal Scientific Center for Agroecology of the Russian Academy of Sciences. The study was conducted from 2022 to 2024. Generative productivity was studied by collecting seeds and assessing their morphological characteristics and viability. DNA was isolated using the CTAB method from the leaf blade, followed by assessment of concentration and quality at wavelengths A260/A280 using a SpectrostarNano spectrophotometer (BMG Labtech, Germany). Results. It was revealed that in G. triacanthos, the seed quality during the study period was at a higher level (96.82%) than in Gleditsia caspica, where these values are within 66,24 %. High germination was recorded in G. triacanthos seeds (up to 92%). In all species, the germinal root develops the fastest. Conclusion. The results of genotyping allowed us to identify both common UBC 873, UBC 890, and variable UBC 826, loci characteristic of representatives of the entire Gleditsia generic complex. A higher polymorphism of G. triacanthos according to the UBC 826 marker was also revealed compared to other representatives of the genus Gleditsia, which is consistent with better morphological characteristics and drought resistance. EDN: GFOGOW
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