Anthrahydroquinone-2,6-Disulfonate Restores Lung Function in COPD Through Keap1/Nrf2 Pathway Activation

Abstract

Zhao Li,1– 4,* Jun Chen,5,* Jiadong Zhang,1– 3,* Nan Li,1– 3 Yang Yi,1– 3 Hanjing Lu,1– 3 Min Li,1– 3 Rui Liu,1– 3 Yuanpeng Chen,1– 3 Xiaoran Liu1 1College of Emergency Trauma, Hainan Medical University, Haikou, Hainan, People’s Republic of China; 2Key Laboratory of Hainan Trauma and Disaster Rescue, the First Affiliated Hospital of Hainan Medical University, Haikou, Hainan, People’s Republic of China; 3Key Laboratory of Emergency and Trauma Ministry of Education, Hainan Medical University, Haikou, Hainan, People’s Republic of China; 4Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, Hainan, People’s Republic of China; 5Danzhou People’s Hospital, Danzhou, Hainan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaoran Liu, College of Emergency Trauma, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan, 571199, People’s Republic of China, Tel [phone], Email [email protected]: Chronic obstructive pulmonary disease (COPD), the third leading cause of death globally, is strongly driven by oxidative damage, contributing to lung function decline and high mortality. Despite advances in understanding oxidative stress in COPD pathogenesis, effective strategies to mitigate oxidative damage and normalize lung function remain a challenge. Anthrahydroquinone-2,6-disulfonate (AH2QDS) has potent antioxidant properties, but its ability to alleviate persistent airway oxidative damage and normalize lung function in COPD patients is not fully elucidated. This study aimed to investigate the therapeutic effects of AH2QDS on COPD by exploring its mechanisms involving the Keap1-Nrf2 pathway via in vivo and in vitro models.Methods: This study induced COPD-like pathology by using LPS in combination with CS / CSE, establishing both in vivo and in vitro models to evaluate the effects of AH2QDS on lung function, histopathology, oxidative stress markers, levels of inflammatory cytokines, and apoptosis. Molecular docking predicted the interaction between AH2QDS and Keap1-Nrf2 proteins, which was confirmed by immunoblotting.Results: AH2QDS treatment significantly mitigated lung tissue injury and restored lung function parameters (MMEF, PEF) in COPD model rats. This treatment upregulated the expression of antioxidant enzymes (SOD, CAT, and GSH-PX) and reduced the levels of inflammatory cytokines (TNF-α, IL-6, IL-1β, and IL-33), ROS, and the total number of apoptotic cells (Annexin V+). Following AH2QDS treatment, the expression of the Nrf2 protein was increased, and the expression of its downstream antioxidant proteins, HO-1 and NQO1, was also upregulated. Molecular docking analysis revealed a high binding affinity between AH2QDS and Keap1-Nrf2 protein (binding energy: − 8.0 kcal/mol).Conclusion: AH2QDS promotes the body’s capacity to counteract oxidative damage and reduces inflammatory cytokine levels by activating the Keap1‒Nrf2 pathway, thereby normalizing lung function in COPD.Keywords: COPD, AH2QDS, oxidative stress, lung function, Keap1-Nrf2

Keywords

• COPD
• AH2QDS
• oxidative stress
• lung function
• Keap1-Nrf2.