Expression and Prognostic Value of VEGF-C and Lymphangeogenesis 
in Lung Adenocarcinoma and Squamous Cell Carcinoma

Abstract

Background and objective Vascular endothelial growth factor-C (VEGF-C), a member of the VEGF family, has been proven to be a relatively special VEGF. When binding to its receptor VEGFR-3, it activates lymphangiogenesis, which likely induces lymph node metastasis in tumors. The aim of this study is to characterize the expression and prognostic value of VEGF-C and lymphangiogenesis in lung adenocarcinoma and squamous cell carcinoma. Methods Through immunohistochemistry, the lymph vessel endothelial cells undergoing lymphangiogenesis were stained with podoplanin to detect the expression of VEGF-C and lymphangiogenesis in 98 cases with stage IIIa (N2) lung adenocarcinoma and squamous cell carcinoma. Results The expression rate of the VEGF-C was positively correlated with the lymphatic microvessel density (LMVD; r=0.783, P<0.01). The LMVD was remarkably higher in VEGF-C positive expression group than that in the VEGF-C negative expression group. The expression rate of VEGF-C and lymphangiogenesis in lung adenocarcinoma was significantly higher than that in lung squamous cell carcinoma (P<0.01). Kaplan-Meier analysis showed that the survival rates in patients with positive VEGF-C expression were significantly lower than those in patients with negative VEGF-C expression (P<0.05). Conclusion Lymphangiogenesis rates are significantly higher in lung adenocarcinoma than that in squamous cell carcinoma. VEGF-C expression is an independent prognostic factor of stage IIIa (N2) lung adenocarcinoma and squamous cell carcinoma.

Keywords

• Lung neoplasms
• Vascular endothelial growth factor-C
• Lymphangiogenesis
• Prognosis