Biochar and Melatonin Partnership Mitigates Arsenic Toxicity in Rice by Modulating Antioxidant Defense, Phytochelatin Synthesis, and Down-Regulating the Transporters Involved in Arsenic Uptake

Abstract

Arsenic (As) contamination has significantly increased in recent decades due to anthropogenic activities. This is a serious challenge for human health, environmental quality, and crop productivity. Biochar (BC) is an important practice used globally to remediate polluted soils. Likewise, melatonin (MT) has also shown tremendous results in mitigating metal toxicity and improving crop productivity. Nevertheless, the mechanism of combined BC and MT in alleviating As toxicity in rice (Oryza sativa L.) remains unexplored. In this study, we investigated how As affected rice and how the combined BC and MT facilitated As tolerance. The study comprised a control, As stress (100 mg kg−1), As stress (100 mg kg−1) + BC (2%), As stress (100 mg kg−1) + MT (100 µM) and As stress (100 mg kg−1) + BC (2%) + MT (100 µM). Arsenic significantly decreased rice growth and yield by increasing electrolyte leakage (EL), malondialdehyde (MDA), and hydrogen peroxide (H2O2). Co-applying BC and MT substantially enhanced rice growth and yield by increasing chlorophyll synthesis (48.12–92.42%) leaf water contents (40%), antioxidant activities (ascorbate peroxide: 56.43%, catalase: 55.14%, peroxidase: 57.77% and superoxide dismutase: 57.52%), proline synthesis (41.35%), MT synthesis (91.53%), and phytochelatins synthesis (125%) nutrient accumulation in rice seedlings and soil nutrient availability. The increased rice yield with BC + MT was also linked with reduced H2O2 production, As accumulation, soil As availability, and an increase in OsAPx6, OsCAT, OsPOD, OsSOD OsASMT1, and OsASMT2 and a decrease in expression of OsABCC1. Biochar + MT enhanced residual OM- and Fe, ((Fe2As) and Mn (Mn3(AsO4)2) bound forms of As leading to a substantial increase in rice growth and yield. Thus, the combination of BC and MT is an eco-friendly approach to mitigate As toxicity and improve rice productivity.

Keywords

• arsenic
• gene expression
• melatonin
• rice
• soil health
• yield