Relation on Cryoprotectants and Arrangement of Hydroxyl Groups

Abstract

In order to study the relationship between cryoprotective and its molecular structure, Pyris-diamond DSC was used in the experiment to study the physical properties of seven kinds of cryoprotective agent, including phase transition temperature, the area melting peaks phase, transition enthalpy value and hydration properties. The unfrozen water share of cryoprotectant was calculated by cooling curve of the phase transition temperature and that of the phase transition enthalpy value. We have discovered that there is a positive correlation between the protective effect of cryoprotectant and the linear arrangement of hydroxyl; the more hydroxyl in the linear arrangement, the better protective effect of cryoprotectant. As a result, the protective effect of cryoprotectant adding sorbitol solution is the highest in seven kinds of cryoprotective agent because sorbitol has five hydroxy.

Keywords

• cryopreservation
• hydration properties
• polyalcohols
• hydroxyl