Using Bioinformatics Tools for Identification and Characterization of Transcriptome Derived EST-SSRs in Silver Fir (Abies alba Mill.)

Abstract

Bioinformatics tools have been used to evaluate silver fir de novo assembled 454 transcriptome. A total of 3500 EST-SSRs were detected in the 454 transcriptome of silver fir. Most abundant are tri-nucleotide SSRs being followed by tetra- SSRs and di- SSRs. In addition, we determined the density, frequency, average length and average repeat number of EST-SSRs in the 454 transcriptome of silver fir.

Keywords

• Bioinformatic tools, transcriptome, EST-SSRs, silver fir