DETERMINATION OF BINDING PARAMETERS OF 2-(FERROCENYLMETHYL-AMINO)BENZONITRILE AND 3-(FERROCENYLMETHYLAMINO)BENZO-NITRILE WITH 2,2-DIPHENYL-1-PICRYLHYDRAZYL FREE RADICAL

Abstract

Spectrophotometrical technics based assay were developed for the calculation of binding parameters like binding constant, ratio of binding constants and binding free energy of two potential antioxidants 2-(ferrocenylmethylamino)benzonitrile (2FMAB) and 3-(ferrocenylmethylamino)benzonitrile (3FMAB) with 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH). 3FMBA shows highest binding constant and highest binding free energy (2.28×10-5 M-1) and (30.06 KJmol-1) respectively. Binding free energy of DPPH-3FMAB is three times greater than that of the standard antioxidant ascorbic acid (10.93 KJmol-1).

Keywords

• ferrocene derivatives
• DPPH
• binding parameters
• binding free energy
• binding constant.