In Vitro Antibacterial Activity of Averrhoa bilimbi Leaves Ethanol Extract Against Salmonella typhi

Abstract

Introduction: Some native plants in Indonesia have potential effects on health and medication. This study aimed to determine the potential antibacterial effect of Averrhoa bilimbi (A. bilimbi) leaves ethanol extract against Salmonella typhi (S. typhi) bacteria. Methods: This was an experimental study. The antibacterial potency of A. bilimbi leaves was measured against S. typhi in vitro. Different concentrations of the leaves’ ethanol extract were prepared. The minimum inhibitory concentration (MIC) was evaluated by a macro-dilution method using Mueller Hinton broth. The minimum bactericidal concentration (MBC) value was observed by subculturing the specimen from the previous dilution tube to the nutrient agar. Results: The MIC of A. bilimbi leaves ethanol extract against S. typhi bacteria could not be determined because all the treatment tubes’ colors were dark and turbid. Meanwhile, the MBC value was at 500 mg/ml. Conclusion: A. bilimbi leaves extract had potential bactericidal effects against S. typhi with a MIC value that could not be determined.

Keywords

• antibacterial activity
• averrhoa bilimbi leaves
• salmonella typhi bacteria
• tropical disease
• typhoid fever