A total of 768 molecular markers were developed for Ipomoea batatas (L.) Lam., consisting of 689 simple sequence repeats (SSRs) and 79 single nucleotide polymorphisms (SNPs). All the markers were distributed across the sweet potato genome, averaging 51 markers per chromosome. The markers were tested on DNA samples from five cultivars of I. batatas, assessing their amplification efficiency and polymorphism. Here we provide the primer sequences tested, their chromosome locations, the analysis including amplicon sizes, and highlight 92 that showed polymorphism between Beauregard and Tanzania. This dataset offers valuable resources for constructing high-resolution linkage maps and facilitates advanced genetic studies and breeding programs in sweetpotato.
