On-Site Dual Detection of Airborne Acinetobacter baumannii and Its Carbapenem-Resistant Gene blaOXA-23 Using a One-Pot Visual LAMP-CRISPR/Cas12a-Based Platform

Huijun Lu; Tong Zhang; Wei Huang; Jinhui Zhu; Haoran Qin; Xi Chen; Wang Zhao; Guodong Sui

doi:10.3390/microorganisms13050976

Microorganisms (Apr 2025)

On-Site Dual Detection of Airborne Acinetobacter baumannii and Its Carbapenem-Resistant Gene blaOXA-23 Using a One-Pot Visual LAMP-CRISPR/Cas12a-Based Platform

Huijun Lu,
Tong Zhang,
Wei Huang,
Jinhui Zhu,
Haoran Qin,
Xi Chen,
Wang Zhao,
Guodong Sui

Affiliations

Huijun Lu: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China
Tong Zhang: Department of Clinical Laboratory, Shanghai East Hospital, School of Medicine, Tong Ji University, Shanghai 200120, China
Wei Huang: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China
Jinhui Zhu: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China
Haoran Qin: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China
Xi Chen: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China
Wang Zhao: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China
Guodong Sui: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China

DOI: https://doi.org/10.3390/microorganisms13050976
Journal volume & issue: Vol. 13, no. 5
p. 976

Abstract

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Acinetobacter baumannii (A. baumannii), a very common pathogen, poses a significant public health threat due to its antibiotic resistance and long survival in healthcare environments. Both A. baumannii and carbapenem-resistant A. baumannii (CRAB) can spread through the air, increasing infection risks. Therefore, monitoring their presence in the air is of great significance, especially in hospitals. Herein, we developed a Chelex-100-LAMP-CRISPR/Cas12a (CLC) platform including DNA release and nucleic acid test. Combined with a wet cyclone sampler, the platform can detect airborne A. baumannii and its most common carbapenem-resistant gene, blaOXA-23, within 70 min. This CLC platform has also been proven to have a detection limit of 6 × 102 CFU of CRAB per test through simulated air samples. Moreover, this platform was also used to test five actual air samples from a tertiary hospital, and the results achieved perfect concordance with sequencing data, validating the platform’s accuracy and reliability. Therefore, the CLC platform showed great potential for the rapid, on-site detection of airborne A. baumannii and its carbapenem-resistant gene blaOXA-23, offering a valuable tool for infection control in healthcare environments.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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