Effect of SV2B Overexpression on Biological Behavior of Glioblastoma and Its Mechanism

Abstract

ObjectiveTo analyze the effect of SV2B overexpression on the growth, invasion, and apoptosis of glioblastoma cells, and to explore its potential mechanism. MethodsWe transfected glioblastoma u87 and u251 cells with lentivirus as SV2B overexpression group. And blank control group was set up. The effects of SV2B overexpression on the proliferation, migration, and invasion of u87 and u251 cells were detected by CCK-8 assay, cell scratch assay, Transwell invasion, and Transwell migration assay. The expression level of SV2B protein was detected by qRT-PCR and Western blot. ResultsCompared with the blank control group, the proliferation, invasion, and migration abilities of u87 and u251 cells in SV2B overexpression group were significantly reduced (P<0.05). ConclusionSV2B overexpression significantly inhibits the proliferation, invasion, and migration abilities of glioblastoma cells.

Keywords

• glioblastoma
• sv2b
• tumor prognosis
• malignant progression