Generation of six induced pluripotent stem cell (iPSC) lines from two patients with amyotrophic lateral sclerosis (NUIGi043-A, NUIGi043-B, NUIGi043-C, NUIGi044-A, NUIGi044-B, NUIGi044-C)

Meimei Yang; Min Liu; Alice Vajda; Timothy O'Brien; David Henshall; Orla Hardiman; Sanbing Shen

doi:10.1016/j.scr.2019.101558

Stem Cell Research (Oct 2019)

Generation of six induced pluripotent stem cell (iPSC) lines from two patients with amyotrophic lateral sclerosis (NUIGi043-A, NUIGi043-B, NUIGi043-C, NUIGi044-A, NUIGi044-B, NUIGi044-C)

Meimei Yang,
Min Liu,
Alice Vajda,
Timothy O'Brien,
David Henshall,
Orla Hardiman,
Sanbing Shen

Affiliations

Meimei Yang: Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland Galway, Galway, Ireland; FutureNeuro Research Centre, Royal College of Surgeons in Ireland, Dublin D02, Ireland
Min Liu: Department of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, China
Alice Vajda: Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland
Timothy O'Brien: Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland Galway, Galway, Ireland
David Henshall: FutureNeuro Research Centre, Royal College of Surgeons in Ireland, Dublin D02, Ireland
Orla Hardiman: FutureNeuro Research Centre, Royal College of Surgeons in Ireland, Dublin D02, Ireland; Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland; Correspondence to: Orla Hardiman, FutureNeuro Research Centre, Royal College of Surgeons in Ireland, Dublin D02, Ireland; Academic Unit of Neurology, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland.
Sanbing Shen: Regenerative Medicine Institute (REMEDI), School of Medicine, National University of Ireland Galway, Galway, Ireland; FutureNeuro Research Centre, Royal College of Surgeons in Ireland, Dublin D02, Ireland; Correspondence to: Sanbing Shen, Regenerative Medicine Institute, School of Medicine, Biomedical Science Building BMS-1021, Dangan, National University of Ireland, Galway, Ireland.

DOI: https://doi.org/10.1016/j.scr.2019.101558
Journal volume & issue: Vol. 40

Abstract

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In this study, we generated 6 induced pluripotent stem cell (iPSC) lines derived from dermal fibroblasts of patients with sporadic amyotrophic lateral sclerosis (sALS). The fibroblasts were reprogrammed using non-integrating Sendai viruses containing four reprogramming factors OCT3/4, SOX2, KLF4 and C-MYC. The iPSC lines displayed normal molecular karyotype, expressed pluripotency markers and were capable of differentiating into three embryonic germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.sciencedirect.com/journal/stem-cell-research

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