Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting

Abstract

Summary: Fibro/adipogenic progenitors (FAPs) are mesenchymal progenitors that support muscle stem cell activation and modulate the extracellular matrix to facilitate muscle regeneration. Here, we present a protocol for the isolation of FAPs from mouse skeletal muscle. We describe steps for performing enzymatic digestion, mechanical dissociation, and magnetic-activated cell sorting (MACS). This protocol enables the isolation of FAPs with high yield and purity, facilitating research on muscle disorders and advancing muscle regeneration strategies. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Biology
• Developmental biology
• Genetics
• Molecular Biology