Aneuploidy and genome organization in sperm nuclei of river buffalo and cattle detected by fluorescence in situ hybridization (FISH) and chromosome microdissection

Abstract

New technologies in molecular cytogenetics have remarkably facilitated the visualization of whole chromosomes or subchromosomal regions in interphase nuclei. Consequently, both somatic and sperm nuclei have been under investigation, in order to study the territorial organization of genetically ‘active’ versus ‘inactive’ genomes. Purpose of the present study was to investigate whether in river buffalo sperm nuclei, chromosomes X-, Y- and 5p (corresponding to chromosome 29 in cattle) maintain or change their relative position compared to cattle.

Keywords

• chromosome, aneuploidy, sperm architecture, FISH